Start with the 07:30 FlixBus bus from Vienna to Vienna West, about 15 min. Transfer in Vienna West, then take the 09:15 FlixBus bus on to Vienna Matzleinsdorferplatz (10 min). Transfer in Vienna, then take the 18:25 (+1d) Ryanair flight on to Prague (5h 35m). You arrive in Prague around 00:00 (+1d). In total it's roughly EUR 93 over 15h, 3 transfers.
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Opens Omio — enter your date to compare trains and buses for this route; the price is finalised at checkout.
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Fly into Vienna, then continue to Prague by bus (15 min) — routing via Vienna keeps the trip simple. Bus (FlixBus, 15min) + Bus (FlixBus, 10min) via Vienna West + Flight (Ryanair, 5h 35min) via Vienna.